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HansaBioMed/BrdU Cell Proliferation Assay Kit (200 reactions)/HBM-K306-200/1 Ea
BrdUCellProliferationAssayKit-200reactions
KitSummary:
•Detectionmethod-Absorbance(450nm).
•Applications-Detectionandquantificationofcellproliferationinducedbygrowthfactors,cytokines,mitogens,andnutrients.
•Analysisofcytotoxicandcytostaticcompoundssuchasanticancerdrugs,toxicagentsandotherpharmaceuticals.
•Determinationoftheinhibitoryorstimulatoryeffectsofvariouscompoundsoncellproliferation.
SampleType:
•AdherentandsUSPensioncells.
Features&Benefits:
•Convenient&non-rADIoactive.
•High-throughputsuitable.
•Highly-sensitive:detectaslowas50-100proliferatingcells.
•Suitableforcellproliferation,chemotaxisandcytotoxicity.
KitComponents:
•BrdU(1000X),
•Fixing/DenaturingSolution.
•BrdUDetectionAntibody(300X).
•Anti-mouseHRP-linkedAntibody(2000X).
•AntibodyDiluent.
•WashBuffer(10X).
•TMBSubstrate.
•StopSolution.
Description:
5-bromo-2-deoxyuridine(BrdU)isapyrimidineanalog.ItgetsincorporatedintothenewlysynthesizedDNAofproliferatingcellsinplaceofthymidine.BrdUCellProliferationAssayKitdetectsincorporatedBrdUusingamouseanti-BrdUantibody.Ananti-mouseHRP-linkedsecondaryantibodyisusedtodetecttheanti-BrdUantibodyboundtoBrdU,whichisfollowedbyadditionofTMB(aHRPsubstrate).TheextentofcolordevelopmentisproportionaltothequantityofBrdUincorporatedintothecellsandcanbeuseddirectlyasanindicatorofcellproliferation.Comparedtoothercellproliferationassays,thiskitdetectsonlytheproliferatingcellsandnottheseededcells.Thishighlysensitive,non-radioactivekitdetectsaslessas50-100proliferatingcells.
StorageConditions:-20°C.
ShippingConditions:gelpack.
USAGE:ForResearchUseOnly!NotForUseinHumans.
