Live-DeadCellStainingKit-100reactions

KitSummary:

•Detectionmethod-Fluorescentmicroscopy(Ex/Em488/518nm)todetectstaineslivecells;(Ex/Em488/615)todetectstaineddeadcells.

•Sampletype-Cellculture(adherentandsUSPensioncells).

•Speciesreactivity-Mammalian,

•Applications-Stainedliveanddeadcellscaneasilybevisualizedbyfluorescencemicroscopy.

Features&Benefits:

•Simpleprocedure;takeslessthan20minutes.

•Fastandconvenient.

Kitcomponents:

•SolutionA(1mMLive-Dye).

•SolutionB(1.0mg/mlPI).

•StainingBuffer.

Description:

Distinguishingbetweenliveanddeadcellsisveryimportantforinvestigationofgrowthcontrolandcelldeath.TheLive-DeadCellStainingKitprovidestheready-to-usereagentsforconvenientdiscriminationbetweenliveanddeadcells.ThekitutilizesLive-Dye™,acell-permeablegreenfluorescentdye(Ex/Em=488/518nm),tostainlivecells.Deadcellscanbeeasilystainedbypropidiumiodide(PI),acellnon-permeableredfluorescentdye(Ex/Em=488/615).Stainedliveanddeadcellscanbevisualizedbyfluorescencemicroscopyusingaband-passfilter(detectsFITCandrhodamine).Thekitprovidessufficientreagentsfor100stainingsusing24-wellplate.

StorageConditions:-20°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.