Xanthine/HypoxanthineColorimetric/FluorometricAssayKit.

SensitiveAssay,HTS.

KitSummary:

•Detectionmethod-Absorbance(570nm)orFluoroescence(Ex/Em=535/587nm)

•Application-ThekitcandetectXanthine/Hypoxanthinelevelsaslowas0.4µMinvariousBIOLOGicalsamples.

Sampletype:

•BodyFluids:serum,plasma,urineetc.

•Animaltissues:liver,muscle,heartetc.

•Cellculture:adherentorsUSPensioncells.

•Cellandtissueculturesupernatant.

Features&Benefits:

•Simpleprocedure;takes~40minutes

•Rapid,sensitiveandhigh-throughputadaptable

•KitcontainsallnecessaryreagentsforaccuratemeasurementofXanthine/Hypoxanthinelevels.

KitComponents:

•XanthineAssayBuffer

•OxiRedTMProbe(inDMSO)

•XanthineEnzymeMix(Lyophilized)

•Developer(Lyophilized)

•XanthineStandard(Lyophilized)

Description:

Xanthine,acatabolicproductofpurinemetabolism,ispresentinbodyfluids,muscletissueandcertainplants.Structurallylikecaffeine,Xanthinehasastimulanteffectandisusedclinicallytotreatthecongestivediseasessuchasasthmaandchronicobstructivepulmonarydisease.XanthineismetabolizedintouricacidandsuperoxidebyXanthineoxidase.Xanthineoxidasedeficiencycausestheraregeneticdisorder-Xanthinuria,andleadstoXanthineaccumulationinurineandblood,whichultimatelyprogressestorenalfailure.RecentstudiesshowthatXanthinelevelsareelevatedfollowingischemicinjury,thusXanthinecanserveasausefulMarkerfortissuehypoxia.EarlydetectionofXanthinealterationinbiologicalfluidsiscrucialformetabolicstudiesandfordiagnosticandtherapeuticmonitoring.InHansABIoMed"sXanthine/HypoxanthineAssaykit,Xanthine/HypoxanthineisspecificallyoxidizedbytheXanthineEnzymeMixtoformanintermediate,whichreactswithDeveloper&Probetoformaproductthatcanbemeasuredcolorimetrically(λ=570nm)orfluorometrically(Ex/Em=535/587nm).Xanthine/HypoxanthineAssaykitisrapid,simpleandsensitive.Thishigh-throughputsuitableassaykitcandetectXanthinelevelsaslowas0.4µMinvariousbiologicalsamples.

StorageConditions:-20°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.