Myeloperoxidase(MPO)FluorometricActivityAssayKit.

HighlySensitiveAssay,HTS

KitSummary:

•Detectionmethod-Fluorescence(Ex/Em=485/525nm)

•Sampletype-CellandTissueculturesupernatants,urine,plasmaandserum,aswellasmanyotherBIOLOGicalfluidsetc.

•Speciesreactivity-Mammalian.

Features&Benefits:

•Simpleprocedure

•Fastandconvenient

•SensitiveassaysformeasuringMPOaslowas0.5μUperwell.

KitComponents:

•AssayBuffer

•MPOSubstrateStock

•MPOProbe

•FluoresceinStandard(1mM)

•MPOPositiveControl

Description:

Myeloperoxidase(MPO)isaperoxidaseenzyme(EC1.11.1.7)mostabundantlypresentinneutrophilgranulocytes.Itisagreenhemoproteinfoundinneutrophilsandmonocytesthatcatalyzesthereactionofhydrogenperoxideandhalideionstoformcytotoxicacidsandotherintermediatesthatplayaroleintheoxygen-dependentkillingoftumorcellsandmicroorganisms.Itshemepigmentcausesthegreencolorinsecretionsrichinneutrophils,suchaspusandsomeformsofmucus.FurThermore,itcanoxidizetyrosinetoatyrosylrADIcalusinghydrogenperoxideasanoxidizingagent.IntheMPOAssayKit,MPOcatalyzestheproductionofNaClOfromH₂O₂andNaCl.Subsequently,NaClOwillreactstoichiometricallywithAminophenylfluorescein(APF)togeneratefluorescein,whichhasastrongfluorescenceandcanbedetectedatEx/Em=485/525nm.Thekitprovidesarapid,simple,sensitive,andreliabletestsuitableasahighthroughputassayofMPOactivity.ThiskitcanbeusedtodetectMPOactivityaslowas0.5μUperwell.

StorageConditions:-20°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.