AspartateAminotransferase(ASTorSGOT)ActivityColorimetricAssayKit.

SensitiveAssay,HTS

KitSummary:

•Detectionmethod-Absorbance(450nm)

•Sampletype-CellandTissueculturesupernatants,urine,plasmaandserum,aswellasmanyotherBIOLOGicalfluidsetc.

•Speciesreactivity-Mammalian

•Application-ThisassaydetectsAST/SGOTwithadetectionlimitof10mUperwell.

Features&Benefits:

•Simpleprocedure

•Fast,reliableandconvenient

•SensitiveassaysformeasuringAST/SGOTactivityinvariousbiologicalsamplesandthekitisalsosuitableforhighthroughputanalyses.

KitComponents:

•ASTAssayBuffer

•ASTEnzymeMix

•Developer

•ASTSubstrate

•GlutamateStandard(0.1M)

•ASTPositiveControl

Description:

Aspartateaminotransferase(AST),alsoknownasGlutamate-oxaloacetatetransaminase(GOT)isatransaminase(EC2.6.1.1)similartothemoreliverspecificalaninetransaminase(ALT).Althoughcommonlyincludedclinicallyaspartofadiagnosticliverfunctiontest,ASThasabroaderclinicalutilitysinceitmayalsobeelevatedindiseasesaffectingotherorgans,suchastheheartormusclesinmyocardialinfarction,alsoinacutepancreatitis,acutehemolyticanemia,severeburns,acuterenaldisease,musculoskeletaldiseasesandtrauma.Itcatalyzesthereaction:

Aspartate+⍺-Ketoglutarate⇌Oxaloacetate+Glutamate.

Diagnostically,itisalmostalwaysmeasuredinunits/liter(U/L).InASTAssayKit,anaminogroupistransferredoffromaspartateto⍺-ketoglutarate.TheproductsofthisreversIBLetransaminationreactionareoxaloacetateandglutamate.Theglutamateisdetectedinareactionthatconcomitantlyconvertsanearlycolorlessprobetocolor(λmax=450nm).Thekitprovidesarapid,simple,sensitiveandreliabletestsuitableasahighthroughputactivityassayofASTwithadetectionlimitof10mUperwell.

StorageConditions:-20°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.