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HansaBioMed/ExoTEST Ready to Use Kit for Overall Exosome capture and quantification from human urine (transparent plate)
ExoTESTisa sandwichenzyme-linkedimmunoassayforin-vitroexosomequantificationinhumanurine.
Sampletype | Urine |
Startingmaterial | Upto100ulofBIOLOGicalsamplecanbeloadedperwell. Unfractionatedplasmasamplecanbedirectlyusedforcapture. Concentratedurinesamplesarerecommendedpriorcaptureaccordingtooursuggestedprotocol. |
Assaytype | Sandwichenzyme-linkedimmunoassay. |
Sensitivity | Thedetectionlimitofanassayislowerthan0.35ugofoverallexosomewhichisanequivalentoflessthan50pgoftargetedexosomesprotein. |
Productoverview | ExoTESTReadytoUseKitistheidealplatformforcapturingandthequantifyingexosomesfrombiologicalsamplessuchasplasmaandurine. ExoTESTReadytoUseKitfeaturespre-coatedELISAplatewithprimaryantibodiesdetectiondirectedagainstexosome-specificMarkers,namelyα-CD9.Secondaryantibodiesandsubstratereagentsareincludedinthekit. Exosomestandardsforassaycalibrationarealsoincludedinthekit.ExosomestandardsarepurifiedbyultracentrifugationandMICROFILtration,quantifiedforoverallproteincontentandexosomenumberandthenlyophilizedforlong-termstorage. ExoTESTreadytousekitsarereliable,easytouse,worksonavarietyofcommerciallyavailabledetectiontechnologies. |
Primaryantibody | α-CD9mouseantibody |
Exosomestandard | Lyophilizedexosomesfromurineofhealthydonors(see details). |
Assayprotocol | Kitcontainsallthereagentsnecessaryforexosomecaptureandproteintargeteddetection. Assaycalibration:Kitcontainsexosomestandardsforassaycalibration.Dependedonthesampletypeused,kitmayincludehumanplasma,serumorurineexosomes.Allexosomestandardsarelyophilizedandquantifiedforoverallproteincontentandexosomesnumber. Thisstandardsenablebothabsoluteandrelativequantificationexosomesinanalyzedsamples. |
Shipmentandstorage | Allthereagentsareshippedat4Candstorageconditionsasrecommendedintheproductinsert. |
Expirationdate | 12months. |
Application:
ExosomecaptureandquantificationusingtheExoTESTensuresdose-dependentexosomedetectioninavarietyofsamples.Thequantificationcanbebothrelativeandabsoluteastheassaycalibrationenablesthecalculationoftheapproximateexosomequantityforeachsample.
Comprehensiveexosomeprofilingbyimplementingdifferentcombinationsofcaptureanddetectionantibodies.ExoTESTcanbecustomizedforassessingmultipleantigensinatotalexosomepopulationwithinasample.Informativeproteinprofilesforthesamplesofinterestcanbecombinedwithsubsequentnucleicacid(mRNAormiRNA)extractionanddownstreamanalysisfromcapturedexosomes.
SpecificdiagnosticapplicationsusingtheExoTESTallowsthequantitativeassessmentoftotalcirculatingexosomesandspecificexosomalsub-populations,definedbydifferentiallyexpressedantigensundernormalandpathologicalconditions.
Relatedproducts :
Exosomestandards
RNAextractionkit
Immunocaptureandisolationtools
