ExoTESTisasandwichenzyme-linkedimmunoassayforin-vitroexosomequantificationincellculturesupernatant.
Sampletype | Cellculturesupernatant. |
Startingmaterial | Upto100ulofBIOLOGicalsamplecanbeloadedperwell. Concentratedcellculturesupernatantsamplesarerecommendedpriorcaptureaccordingtooursuggestedprotocol. |
Assaytype | Sandwichenzyme-linkedimmunoassay. |
Sensitivity | Thedetectionlimitofanassayislowerthan0.35ugofoverallexosomewhichisanequivalentoflessthan50pgoftargetedexosomesprotein. |
Productoverview | ExoTESTReadytoUsetestKitistheidealplatformforcapturingandthequantifyingexosomesfromcellculturesupernatant. ExoTESTReadytoUsetestKitfeaturespre-coatedELISAplatewithprimaryantibodiesdetectiondirectedagainstexosome-specificMarkers,namelya-CD9.Secondaryantibodiesandsubstratereagentsareincludedinthekit. Exosomestandardsforassaycalibrationarealsoincludedinthekit.ExosomestandardsarepurifiedbyultracentrifugationandMICROFILtration,quantifiedforoverallproteincontentandexosomenumberandthenlyophilizedforlong-termstorage. ExoTESTReadytoUsetestKitsarereliable,easytouse,worksonavarietyofcommerciallyavailabledetectiontechnologies. |
Primaryantibody | a-CD9mouseantibody. |
Exosomestandard | LyophilizedexosomesfromCOLO-1Cellculturesupernatant(seedetails). |
Assayprotocol | Kitcontainsallthereagentsnecessaryforexosomecaptureandproteintargeteddetection. Assaycalibration:Kitcontainsexosomestandardspreparedfrompurifiedfromproprietaryhumancelllineforassaycalibration.Allexosomestandardsarelyophilizedandquantifiedforoverallproteincontentandexosomesnumber. HansABIoMedalsoprovidesarangeofexosomestandardspurifiedfromdifferenttypesofhumancellsthatcanbepurchasedseparatelyorincludedincustomkitsinordertomeettheneedfordetectionofspecificexosomemarkers. |
Shipmentandstorage | Allthereagentsareshippedat4Candstorageconditionsasrecommendedintheproductinsert. |
Expirationdate | 12months. |