ExoTESTisasandwichenzyme-linkedimmunoassayforin-vitroexosomequantificationinhumanserum,plasmaandurine.
Sampletype | Plasma,serum,urine. |
Startingmaterial | Upto100ulofBIOLOGicalsamplecanbeloadedperwell. Unfractionatedplasmasamplecanbedirectlyusedforcapture. Concentratedurinesamplesarerecommendedpriorcaptureaccordingtooursuggestedprotocol. |
Assaytype | Sandwichenzyme-linkedimmunoassay. |
Sensitivity | Thedetectionlimitofanassayislowerthan0.35ugofoverallexosomewhichisanequivalentoflessthan50pgoftargetedexosomesprotein. |
Productoverview | ExoTESTReadytoUseKitistheidealplatformforcapturingandthequantifyingexosomesfrombiologicalsamplessuchasplasma,serum,urineandcellculturesupernatant. ExoTESTReadytoUseKitfeaturespre-coatedELISAplatewithprimaryantibodiesdetectiondirectedagainstexosome-specificMarkers,namelyα-CD9.Secondaryantibodiesandsubstratereagentsareincludedinthekit. Exosomestandardsforassaycalibrationarealsoincludedinthekit.ExosomestandardsarepurifiedbyultracentrifugationandMICROFILtration,quantifiedforoverallproteincontentandexosomenumberandthenlyophilizedforlong-termstorage. ExoTESTreadytousekitsarereliable,easytouse,worksonavarietyofcommerciallyavailabledetectiontechnologies. |
Primaryantibody | α-CD9mouseantibody. |
Exosomestandard | LyophilizedexosomesfromCOLO-1Cellculturesupernatant(seedetails). |
Assayprotocol | Kitcontainsallthereagentsnecessaryforexosomecaptureandproteintargeteddetection. Assaycalibration:kitcontainsexosomestandardsforassaycalibration.Allexosomestandardsarelyophilizedandquantifiedforoverallproteincontentandexosomesnumber. Thisstandardsenablebothabsoluteandrelativequantificationexosomesinanalyzedsamples. |
Shipmentandstorage | Allthereagentsareshippedat4Candstorageconditionsasrecommendedintheproductinsert. |
Expirationdate | 12months. |