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HansaBioMed/从尿液和细胞培养基中分离循环和EV相关DNA（20次反应）/HBM DNAc UC-
EXO-DNAc-UC:IsolationofcirculatingandEV-associatedDNAfromurineandcellculturemedia.
Sampletype | Cellculturemediaandurine |
Startingmaterial | Startingfrom1mlofcellmediaperreaction.Startingfrom5mlofurine. |
Specifications | ThiskitprovideshighcapacityforpurificationofcirculatingandEVassociatedDNA.PelletafterisolationofEVsandcirculatingDNAcanbetreatedwithDnasetoeliminatecellfreecirculatingDNA.AfterDnasetreatmentDNAextractionfromEVsproceedsfollowingtheidenticalprotocol.EXO-DNAcprovidesanappropriatedDNAconcentratorforconcentratingtheyieldandincreasingthepurityoftheDNAtothelevelsrequiredfordigitalPCRanalysis. |
Productoverview | EVsareisolatedquicklywithDNA-Prepreagent. Exosomestandardsforpositivecontrolareincludedinthekit(1vial,100ug). ThekitcontainsallthereagentsnecessaryforDNApurification,includingDNApurificationcolumnsandDNAconcentratorcolumns.Dnaseisnotprovided. |
Exosomestandard | ChosenbytheuserinHBMLyophilizedExosomeStandardslist(seedetails). |
Shipmentandstorage | Allthereagentsareshippedat4Candstorageconditionsasrecommendedintheproductdatasheet. |
Expirationdate | 12months. |
Applications | DirectEVscaptureandDNApurificationfromplasmaandserumwithouttimeconsumingEVpurificationsteps. IsolationofgenomicEV-associatedDNAbyDnasetreatment. Profilingofcirculatingand/orEV-associatedgenomicDNA. DetectionofactionablemutationsbydigitalPCRanalysis. IsolatedDNAissuitableforNGS. |
Relatedproducts :
Exosomestandards
RNAextractionkit
Exosomeisolationtools
