QuickCellProliferationAssayKit-2500reactions

KitSummary:

•Detection-Absorbance(420-480nm).

•Sampletype-Cellculture(adherentandsUSPension).

•Speciesreactivity-Mammalian.

•Applications-Theassaymeasurescellproliferationinresponsetogrowthfactors/cytokines/mitogens/andnutrientsetc.

Features&Benefits:

•Simpleone-stepprocedure;takeslessthan4hours.

•Convenient.

•Usesawater-solubletetrazoliumsaltWSTreagent.Themethodissosimplethatrequiresnowashing/harvesting/orsolubilizationstepsandisfasterandmoresensitivethanMTT/XTT/orMTS-basedassays.Theentireassaycanbeperformeddirectlyina96-wellplate.

Kitcomponents:

•WSTReagent(lyophilized).

•ElectroCouplingSolution(ECS).

•StopSolution.

Description:

TheQuickCellProliferationAssayKitIIprovidesbyfartheeasiestandmostsensitivemeansforquantifyingcellproliferationandviABIlity.Theassayisbasedonthecleavageofthetetrazoliumsalttoformazanbycellularmitochondrialdehydrogenase.Theamountofthedyegeneratedbyactivityofdehydrogenaseisdirectlyproportionaltothenumberoflivingcells.Theformazandyeproducedbyviablecellscanbequantifiedbymulti-wellspectrophotometer(microtiterplatereader)bymeasuringtheabsorbanceofthedyesolutionat440nm.Theassaycanbeusedformeasurementofcellproliferationinresponsetogrowthfactors,cytokines,mitogens,andnutrients,etc.Itcanalsobeusedfortheanalysisofcytotoxiccompoundslikeanticancerdrugsandmanyothertoxicagentsandpharmaceuticalcompounds.Thenewmethodissosimple,justadd-and-read,requiringnowashing,noharvesting,andnosolubilizationsteps.Itisfaster,stable,andmoresensitivethanMTT,XTT,MTSbasedassays.Theassaycorrelateswellwiththe[3H]-thymidineincorporationassay.

StorageConditions:-20°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.