SenescenceDetectionKit-250reactions

KitSummary:

•Sampletype-Cellculturecells,tissues.

•Speciesreactivity-Mammalian.

•Kitsize-250stainings.

•Applications-TheSenescenceDetectionKitisdesignedtohistochemicallydetectspecificsenescenceMarkerindistinctpHinculturedcellsandtissuesections.

Features&Benefits:

•Simpleprocedure;takesaroundthan30minutes.

•Fastandconvenient.

•Thespecifichistochemicalmarkerisonlypresentinsenescentcellsandisnotfoundinpresenescent,quiescentorimmortalcells.

Kitcomponents:

•FixativeSolution(1X).

•X-Gal(150mg,lyophilized).

•StainingSolution(1X).

•StainingSupplement(100X).

Description:

Senescenceisthoughttobeatumorsuppressivemechanismandanunderlyingcauseofaging.Senescencerepresentsanarrestedstateinwhichthecellsremainviable,butnotstimulatedtodividebyserumorpassageinculture.Senescentcellsdisplayincreaseofcellsize,senescence-associatedexpressionofβ-galactosidase(SA-β-Gal)activity,andalteredpatternsofgeneexpression.TheSenescencedetectionkitisdesignedtohistochemicallydetectSA-β-Galactivityinculturedcellsandtissuesections,aknowncharacteristicofsenescentcells.TheSA-β-Galispresentonlyinsenescentcellsandisnotfoundinpresenescent,quiescentorimmortalcells.

StorageConditions:-20°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.