Ready-to-useCellProliferationReagent,WST-1-2500reactions

KitSummary:

•Detectionmethod-Absorbance440nm.

•Speciesreactivity-Mammalian.

•Applications-Measurementofcellproliferationinresponsetogrowthfactors,cytokines,mitogens,andnutrients,etc.Analysisofcytotoxicandcytostaticcompoundssuchasanticancerdrugs,toxicagentsandotherpharmaceuticals.Assessmentofphysiologicalmediatorsandantibodiesthatinhibitcellgrowth.

sampleType:

•Cellcultures(adherentandsUSPension)

Features&Benefits:

•Simpleprocedure.

•Fastandconvenient.

•Theassayisnon-rADIoactive,rapidandmoresensitivethanMTT,XTT,orMTS-basedassays.

•Theentireassaycanbeperformedinthesamemicrotiterplateanddoesnotrequireextrastepslikewashing,harvestingandcellsolubilization.

Kitcomponents:

•WST-1Reagent(inelectroncouplingsolution).

Description:

Theready-to-usecellproliferationreagent,WST-1providesasimpleandaccuratemethodtomeasurecellproliferation,whichisbasedonthecleavageofthetetrazoliumsaltWST-1toformazanbycellularmitochondrialdehydrogenases.Expansioninthenumberofviablecellsresultsinanincreaseintheactivityofthemitochondrialdehydrogenases,whichinturnleadstoincreaseintheamountofformazandyeformed.Theformazandyeproducedbyviablecellscanbequantifiedbymeasuringtheabsorbanceat440nm.Thisnewmethodisnon-radioactive,rapidandmoresensitivethanMTT,XTT,orMTS-basedassays.Theentireassaycanbeperformedinthesamemicrotiterplateanddoesnotrequireextrastepslikewashing,harvestingandcellsolubilization.

StorageConditions:-20°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.