Fraction-PREPCellFractionationKit-50reactions

KitSummary:

•Sampletype-Cellculture(adherentandsUSPension),freshandfrozentissues.

•Speciesreactivity-Mammalian.

•Applications-1-Dor2-Dgel,enzymeactivityassays,gelshiftassay,andWesternblotting.

Features&Benefits:

•Simpleprocedure;takeslessthan1hour.

•Fastandconvenient.

•Thekitisdesignedforserialsamplepreparationoffourdistinctproteinfractionsincludingcytosol/particulate/Cytoskeleton/nuclearfractions,fromonesample.

Kitcomponents:

•CytosolExtractionBuffer(CEB).

•MembraneExtractionBuffer-A(MEB-A).

•MembraneExtractionBuffer-B(MEB-B).

•NuclearExtractionBuffer(NEB).

•DTT(1M).

•ProteaseInhibitorCocktail(lyophilized).

Description:

ThisFractionPREP™CellFractionationsystemisdesignedtoprovidereproducIBLeextractionoffoursubcellularproteinfractions(Cytosol,nucleus,membrane/particulate,andcytoskeletalfractions)fromasinglemammaliansample.Themethodisfastandsimple,needingonly2hoursandnoultracentrifugationinvolved.Allfourproteinfractionsobtainedaresuitableformanydownstreamapplicationssuchas1-Dor2-Dgel,enzymeactivityassays,gelshiftassay,andWesternblotting.

StorageConditions:-20°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.