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HansaBioMed/Mitochondria/Cytosol Fractionation Kit (100 reactions)/HBM-K256-100/1 Ea
Mitochondria/CytosolFractionationKit-100reactions
KitSummary:
•Detectionmethod-Westernblotting,ELISA,orotherassays.
•Sampletype-CellsandTissues.
•Speciesreactivity-Mammalian
•Applications-Effectiveisolationofahighlyenrichedmitochondrialfractionfromcytosolicfractionofmammaliancellsincludingbothapoptoticandnonapoptoticcells.
Features&Benefits:
•Simpleprocedure;takesonly3-4hours.
•Fastandconvenient.
•Thefractionationprocedureissimpleandstaightforward.Noultracentrifugationsarerequired.Notoxicchemicalsareinvolved.
Kitcomponents:
•MitochondriaExtractionBuffer.
•5XCytosolExtractionBuffer.
•DTT(1M).
•ProteaseInhibitorCocktail.
Description:
TheMitochondria/CytosolFractionationKitprovidesuniqueformulationsofreagentsforeffectiveisolationofahighlyenrichedmitochondrialfractionfromcytosolicfractionofmammaliancellsincludingbothapoptoticandnonapoptoticcells.TheenrichedmitochondrialandcytosolicfractionscanbeusedforstudyingapoptoticandsignaltransductionpathwaystodetecttranslocationoffactorsinterestedbetweenthetwofractionsbyWesternblotting,ELISA,orotherassays.Proceduresaresimpleandeasytoperform,noultracentrifugationsandtoxicchemicalsareinvolved.
StorageConditions:-20°C.
ShippingConditions:gelpack.
USAGE:ForResearchUseOnly!NotForUseinHumans.
