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HansaBioMed/Mitochondrial DNA Isolation Kit (50 reactions)/HBM-K280-50/1 Ea
MitochondrialDNAIsolationKit-50reactions
KitSummary:
•Sampletype-Cellculture(adherentandsUSPension),freshandfrozentissues.
•Speciesreactivity-Mammalian.
•Applications-Enzymemanipulations,Southernblotting,cloning,PCRanalysis,andamplifications.
Features&Benefits:
•Simpleprocedure;takeslessthan2hours.
•Fastandconvenient.
•Thekitprovidesuniqueformulationsofbuffersandreagentsforisolatingahighlyenrichedmitochondrialfraction.MitochondrialDNAarethenisolatedusingreagentsincludedinthekit.
Kitcomponents:
•5XCytosolExtractionBuffer.
•MitochondrialLysisBuffer.
•EnzymeBMix(lyophilized).
•TEBuffer.
Description:
Mitochondriaaresemiautonomousorganelleswhichfunctionsinagingprocess,apoptosis,anti-HIVdrugs,andcancers.MitochondrialDNA(mtDNA)hasaveryhighmutationrateandthemutationsonmtDNAappeartoberelatedtocertaindiseasessuchasdiabetes,Alzheimer’sdisease,andmuscledisorders.IsolationandquantificationofmtDNAareoftenrequiredtostudytherelationshipsbetweenthediseasesandmtDNA.TheMitochondrialDNAExtractionKitprovidesconvenienttoolsforisolatingmtDNAfromavarietyofcellsandtissuesinhighyieldandpurity,withoutcontaminationsfromgenomicDNA.ThepurifiedmtDNAcanbeusedforavarietyofstudiessuchasenzymemanipulations,Southernblotting,cloning,PCRanalysis,andamplifications.
StorageConditions:-20°C.
ShippingConditions:gelpack.
USAGE:ForResearchUseOnly!NotForUseinHumans.
