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HansaBioMed/MTS Cell Proliferation Colorimetric Assay Kit (500 reactions)/HBM-K300-500/1 Ea
MTSCellProliferationColorimetricAssayKit-500reactions
KitSummary:
•Detectionmethod-Absorbance(490-500nm).
•Applications-Measurementofcellproliferationinresponsetogrowthfactors,cytokines,mitogensandnutrients.
-Analysisofcytotoxicandcytostaticcompoundssuchasanticancerdrugs,toxicagentsandotherpharmaceuticals.
-Assessmentofphysiologicalmediatorsthatinhibitcellgrowth.
SampleType:
•AdherentandsUSPensioncells.
Features&Benefits:
•Simpleone-stepprocedure;takes~4hours.
•High-throughput.
•Theentireassaycanbeperformeddirectlyina96-wellplateanddoesnotrequirewashing/harvesting/orsolubilizationsteps.
Kitcomponents:
•MTSReagent(inelectrocouplingsolution).
Description:
MTSCellProliferationAssayKitisacolorimetricmethodforsensitivequantificationofviablecellsinproliferationandcytotoxicityassay.ThemethodisbasedonthereductionofMTStetrazoliumcompoundbyviablecellstogenerateacoloredformazanproductthatissolubleincellculturemedia.ThisconversionisthoughttobecarriedoutbyNAD(P)H-dependentdehydrogenaseenzymesinmetabolicallyactivecells.Theformazandyeproducedbyviablecellscanbequantifiedbymeasuringtheabsorbanceat490-500nm.Theassaycanbeusedforthemeasurementofcellproliferationinresponsetogrowthfactors,cytokines,mitogens,andnutrients,etc.Itcanalsobeusedfortheanalysisofcytotoxiccompoundslikeanticancerdrugsandmanyothertoxicagentsandpharmaceuticalcompounds.MTSassayisperformedbyaddingthereagentdirectlyintothecellculturemediawithouttheintermittentsteps,whicharerequiredintheroutineMTTassay.Inaddition,thishigh-throughputassayrequiresnowashingorsolubilizationstepandcanbeperformedin96-wellmicrotiterplate.
StorageConditions:-20°C.
ShippingConditions:gelpack.
USAGE:ForResearchUseOnly!NotForUseinHumans.
