Nuclear/CytosolFractionationKit-100reactions

KitSummary:

•Sampletype-Cellculture(adherentandsUSPension),freshandfrozentissues.

•Speciesreactivity-Mammalian.

•Applications-ThisNuclear/CytosolExtractionKitprovidesacompletesystemthatenablestheseparationofnuclearextractfromthecytoplasmicfractionofmammaliancellsandtissuesThenewkitprovidesasystemthatmaintainsthenuclearandcytoplasmiccompartmentsseparateandintact.Transcriptionalactivity/reporterassays/enzymeactivityassays/Westernblottingandmoredownstreamassays.

Features&Benefits:

•Simpleprocedure;takeslessthan2-3hours.

•Fastandconvenient.

•Theoptimizedreagentsandproceduresprovidedwiththekitallowseparationofnuclearandcytoplasmicextractsfromcellsrelativelyquickwithlittleornocross-contaminations.

Kitcomponents:

•CytosolExtractionBufferA(CEB-A).

•CytosolExtractionBufferB(CEB-B).

•NuclearExtractionBuffer(NEB).

•DTT(1M).

•ProteaseInhibitorCocktail(lyophilized).

Description:

ThisNuclear/CytosolExtractionKitprovidesacompletesystemthatenablestheseparationofnuclearextractfromthecytoplasmicfractionofmammaliancells.Theoptimizedreagentsandproceduresprovidedwiththekitallowseparationofnuclearandcytoplasmicfractionsquicklywithlittleornocross-contaminations.TheextractednuclearandcytoplasmicproteinfractionsarefunctionalandcompatIBLewithdownstreamassayssuchastranscriptionalactivity,RNAsplicing,gelshiftassay,reporterassays,enzymeactivityassays,andWesternblotting.

StorageConditions:-20°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.