ProteasomeActivityAssayKit-100reactions

KitSummary:

•Detectionmethod-Fluorescence(Ex/Em350/440nm).

•Sampletype-Wholecelllysates,nuclearandcytoplasmiclysates.

•Speciesreactivity-Mammalian.

•Applications-Specificallymeasuresproteasomeactivityfromotherproteaseactivitypresentinsamples.

Features&Benefits:

•Simpleone-stepprocedure;takesonly30-60minutes.

•Accurateandeasytouse.

Kitcomponents:

•ProteasomeAssayBuffer.

•ProteasomeSubstrate(InDMSO).

•ProteasomeInhibitor(InDMSO).

•AMCStandard(1mMinDMSO).

•PositiveControl.

Description:

Proteasomesareverylarge(20S,26S)proteinassembliesfoundinboththenucleusandcytoplasmofalleucaryotes(andinsomeprocaryotes).TheyareresponsIBLeforthedegradationandrecyclingofproteinswhichhavebeenpreviouslytaggedwithubiquitin.Suchtaggedproteinsaredegradedintopeptidesapproximately7-8aminoacidslongwhicharesubsequentlyfurtherdegraded.The20Sassemblyisthefunctionalproteasestructurewithchymotrypsin-like,trypsin-likeandcaspase-likeproteaseactivities.ProteasomeActivityAssaytakesadvantageofthechymotrypsin-likeactivity,utilizinganAMC-taggedpeptidesubstratewhichreleasesfree,highlyfluorescentAMCinthepresenceofproteolyticactivity.Thekitalsoincludesapositivecontrol(JurkatCelllysatewithsignificantproteasomeactivity)andaspecificproteasomeinhibitorMG-132whichsuppressesallproteolyticactivityduetoproteasomes.Thispermitsdifferentiationofproteasomeactivityfromotherproteaseactivitywhichmaybepresentinsamples.

StorageConditions:-20°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.