NitricOxideColorimetricAssayKit.

Detectstotalnitrate/nitrite

KitSummary:

•Detectionmethod-Absorbance(540nm)

•Sampletype-CellandTissuelysates,culturemedia,urine,plasmaandserum,aswellasmanyotherBIOLOGicalfluids

•Speciesreactivity-Mammalian

•Applications-HansABIoMed’sNitricOxideColorimetricAssayKitprovidesanaccurate,convenientmeasureoftotalnitrate/nitriteinasimpletwo-stepprocess

Features&Benefits:

•Simpletwo-stepprocedure;takesonly~1-1.5hours

•Fastandconvenient

•HansaBioMed’sNitricOxideColorimetricAssayKitprovidesanaccurate,convenientmeasureoftotalnitrate/nitrite.

KitComponents:

•AssayBuffer

•Enzymecofactor

•Enhancer

•NitrateReductase

•NitrateStandard

•NitriteStandard

•GriessReagentR1

•GriessReagentR2

•MicrotiterPlate

•PlateCover

Description:

Nitricoxide(NO)playsanimportantroleinneurotransmission,vascularregulation,immuneresponseandapoptosis.NOisrapidlyoxidizedtonitriteandnitratewhichareusedtoquantitateNOproduction.TheNitricOxideColorimetricAssayKitprovidesanaccurate,convenientmeasureoftotalnitrate/nitriteinasimpletwo-stepprocess.Thefirststepconvertsnitratetonitriteutilizingnitratereductase.ThesecondstepusesGriessReagentstoconvertnitritetoadeeppurpleazocompound.Theamountoftheazochromophoreaccuratelyreflectsnitricoxideamountinsamples.Thedetectionlimitoftheassayisapproximately1.0nmolenitrite/well,or10µM.

StorageConditions:-20°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.