NitricOxideFluorometricAssayKit.

KitSummary:

•Detectionmethod-Fluorescence(Ex/Em380/461nm)

•Sampletype-CellandTissuelysates,culturemedia,urine,plasmaandserum,aswellasmanyotherBIOLOGicalfluids

•Speciesreactivity-Mammalian

•Applications-Measurementoftotalnitrate/nitriteconcentration.

Features&Benefits:

•Simpleprocedure;takesonly2-3hours

•Fastandconvenient

•Thefluorometrickitprovidesasimpletwo-stepprocessforquantitativemeasurementoftotalnitrate/nitriteproductions.Theassaycanbeeasilydoneina96-wellplate.

KitComponents:

•AssayBuffer

•EnzymeCofactor

•Enhancer

•NitrateReductase

•NitrateStandard

•NitriteStandard

•DANProbe

•SodiumHydroxide

•Microtiterplate

•PlateCover

Description:

Nitricoxide(NO)playsanimportantroleinneurotransmission,vascularregulation,immuneresponseandapoptosis.SinceNOisrapidlyconvertedtonitrite(NO²ˉ)andnitrate(NOᶟˉ),thetotalconcentrationofnitriteandnitrateisusedasaquantitativemeasureofNOproduction.TheNitricOxideFluorometricAssayKitprovidesanaccurateandconvenientmeasurementoftotalnitrate/nitriteconcentrationinasimpletwo-stepprocess.Inthefirststepnitrateisconvertedtonitritebynitratereductase.Inthesecondstep,nitritereactswiththefluorescentprobeDAN(2,3diaminonaphthalene).NaOHenhancesthefluorescentyield.Thefluorescentintensityisproportionaltothetotalnitricoxideproduction.Thekithasbeentestedwithculturemedia,plasma,andtissuehomogenates.

StorageConditions:-20°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.