PeroxidaseActivityColorimetric/FluorometricAssayKit.

HighlySensitiveAssay,HTS

KitSummary:

•Detectionmethod-Absorbance(570nm)orFluorescence(Ex/Em=535/587)

•Speciesreactivity-Mammalian

•Application-Thekithasadetectionlimitof0.1mUperwellviacolorimetricor0.01mUperwellviafluorometricmethod,basedonourunitdefinition.

Features&Benefits:

•Simpleprocedure

•Fastandconvenient

•Theassayissimple,direct,highlysensitiveandhigh-throughputadaptable.

KitComponents:

•AssayBuffer

•OxiRed™Probe(inDMSO)

•H₂O₂Substrate(0.88M)

•HRPPositiveControl

Description:

Peroxidases(ECnumber1.11.1.x)arealargefamilyofenzymesthattypicallycatalyzeareactionoftheform:ROOR"+electrondonor(2e-)+2H+→ROH+R"OH.Formanyoftheseenzymestheoptimalsubstrateishydrogenperoxide,butothersaremoreactivewithorganichydroperoxidessuchaslipidperoxides.Peroxidasescancontainahemecofactorintheiractivesites,oralternatelyredox-activecysteineorselenocysteineresidues.HansABIoMed’sPeroxidaseAssayKitprovidesaconvenientcolorimetricandfluorometricmeanstomeasuretheperoxidaseactivityinBIOLOGicalsamples.InthepresenceofPeroxidase,theOxiRedProbereactswithH₂O₂ina1:1stoichiometrytoproducethered-fluorescentoxidationproduct,resorufin.Theresorufinisquantifiedbycolorimetric(lmax=570nm)orfluorometricmethods(Ex/Em=535/587nm).Theassayissimple,direct,highlysensitiveandhighthroughput-ready.Thedetectionlimitis0.1mUperwellviacolorimetricor0.01mUperwellviafluorometricmethod,basedonourunitdefinition.

StorageConditions:-20°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.