TotalAntioxidantCapacity(TAC)ColorimetricAssayKit.

KitSummary:

•Detectionmethod-Absorbance(570nm)

•Sampletype-CellandTissuelysates,culturemedia,urine,plasmaandserum,aswellasmanyotherBIOLOGicalfluids

•Speciesreactivity-Mammalian

•Applications-AssaymeasuringeitherthecombinationofbothsmallmoleculeandproteinantioxidantsorsmallmoleculesantioxidantaloneinthepresenceofourproprietaryProteinMask.

Features&Benefits:

•Simpleprocedure;takeslessthan2hours

•Fastandconvenient.

KitComponents:

•Cu²ᶧReagent

•AssayDiluent

•ProteinMask

•TroloxStandard

Description:

AntioxidantsplayanimportantroleinpreventingtheformationofandscavengingoffreerADIcalsandotherpotentiallytoxicoxidizingspecies.Therearethreecategoriesofantioxidantspecies:enzymesystems(GSHreductase,catalase,peroxidase,etc.),smallmolecules(ascorbate,uricacid,GSH,vitaminE,etc.)andproteins(albumin,transferrin,etc.).Differentantioxidantsvaryintheirreducingpower.Troloxisusedtostandardizeantioxidants,withallotherantioxidantsbeingmeasuredinTroloxequivalents.MeasurementofthecombinednonenzymaticantioxidantcapacityofbiologicalfluidsandothersamplesprovidesanindicationoftheoverallcapABIlitytocounteractreactiveoxygenspecies(ROS),resistoxidativedamageandcombatoxidativestress-relateddiseases.Insomecases,theantioxidantcontributionofproteinsisdesiredwhereasinothercasesonlythecontributionofthesmallmoleculeantioxidantsisneeded.HansaBioMeddevelopedtheTACAssayKit,whichcanmeasureeitherthecombinationofbothsmallmoleculeantioxidantsandproteinsorsmallmoleculesaloneinthepresenceofourproprietaryProteinMask.Cu²ᶧionisconvertedtoCu+bybothsmallmoleculeandprotein.TheProteinMaskpreventsCu²ᶧreductionbyprotein,enablingtheanalysisofonlythesmallmoleculeantioxidants.ThereducedCu+ionischelatedwithacolorimetricprobegivingabroadabsorbancepeakaround570nm,proportionaltothetotalantioxidantcapacity.

StorageConditions:+4°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.