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HansaBioMed/Ascorbic acid assay Kit II (100 reactions)/HBM-K671-100/1 Ea
AscorbicAcidColorimetricAssayKitII(FRASC).
KitSummary:
•Detectionmethod-Absorbance(545-600nm)
•Sampletype-CellandTissueculturesupernatants,urine,plasmaandserum,aswellasmanyotherBIOLOGicalfluids,growthmediaandfoodproducts
•Speciesreactivity-Mammalian
•Application-Theassaycandetect0.2to20nmolofascorbicacidinvarioussamples.
Features&Benefits:
•Simpleprocedure;takes~30minutestocompletetheassay
•Fastandconvenient
KitComponents:
•FRASCBuffer
•AscorbicAcidProbe
•FeCl3solution
•AscorbateOxidase(lyophilized)
•AscorbicAcidStandard(20µmole).
Description:
AscorbicAcid(VitaminC)playsanimportantroleinmanybiologicalprocesses.Itisapotentanti-oxidant,anti-inflammatory,anti-viralagentandanimmunestimulantandispresentinawidevarietyofbiologicalspecimens.Duetothepresenceofavarietyofotherantioxidantsinbiologicalsamplessuchasserum,mostascorbicacidassaysshowstronginterference.FRASCAssayKitprovidesarapid,simple,andsensitivemeansofdetectingascorbicacidinbiologicalsamplessuchasserumandotherbodyfluids,tissueandcellextracts,growthmediaandfoodproducts.Inthisassay,FeᶟᶧisreducedtoFe²ᶧbyanyantioxidantspresent.Theferrousironischelatedwithacolorimetricprobetoproduceaproductwithastrongabsorbancebandwhichcanbemonitoredbetween545-600nm.Theadditionofascorbateoxidasetoparallelsamplesremovesanyascorbatepresentleavingabackgroundvaluewhichissubtractedfromthetotaltogiveascorbatecontent.Theassaycandetect0.2to20nmolofascorbicacidinvarioussamples.
StorageConditions:-20°C.
ShippingConditions:gelpack.
USAGE:ForResearchUseOnly!NotForUseinHumans.
