CatalaseActivityColorimetric/FluorometricAssayKit.

KitSummary:

•Detectionmethod-Absorbance(570nm)orFluorescence(Ex/Em535/587nm)

•Sampletype-CellandTissueculturesupernatants,urine,plasmaandserum,aswellasmanyotherBIOLOGicalfluids,growthmedium,foodetc.

•Speciesreactivity-Mammalian

•Applications-Thekitdetectshighpico-unitofcatalaseinsamples.

Features&Benefits:

•Simpleprocedure;takes~30-40minutes

•Fastandconvenient

•SensitiveassaysformeasuringCatalaseinvariousbiologicalsamples

KitComponents:

•CatalaseAssayBuffer

•OxiRed™Probe(inDMSO)

•HRP(lyophilized)

•H₂O₂(3%;0.88M)

•StopSolution

•CatalasePositiveControl(lyophilized)

Description:

Catalase(EC1.11.1.6)isaubiquitousantioxidantenzymethatispresentinnearlyalllivingorganisms.Itfunctionstocatalyzethedecompositionofhydrogenperoxide(H₂O₂)towaterandoxygen.CatalaseAssayKitprovidesahighlysensitive,simple,directandHTS-readyassayformeasuringCatalaseactivityinbiologicalsamples.Intheassay,catalasefirstreactswithH₂O₂toproducewaterandoxygen,theunconvertedH₂O₂reactswithOxiRed™probetoproduceaproduct,whichcanbemeasuredat570nm(Colorimetricmethod)oratEx/Em=535/587nm(fluorometricmethod).Catalaseactivityisreverselyproportionaltothesignal.Thekitdetectshighpico-unitofcatalaseinsamples.

StorageConditions:+4°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.