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HansaBioMed/Cytochrome c Releasing Apoptosis Assay Kit (100 reactions)/HBM-K257-100/1 Ea
CytochromecApoptosisAssayKit.
KitSummary:
•Detectionmethod-Westernblotting
•Sampletype-CellsandTissues
•Speciesreactivity-Mammalian
•Applications-Effectivemeansfordetectingcytochromectranslocationfrommitochondriaintocytosolduringapoptosis.Thekitprovidesaneasy-to-useprocedureandreagentsforseparatingamitochondria-enrichedfractionfromcytosol.
Features&Benefits:
•Simpleprocedure;takes~3hours
•Fastandconvenient
•CytochromecreleasingfrommitochondriaintocytosolisthendeterminedbyWesternblottingusingthecytochromecantibodyprovidedinthekit.Theprocedureissimpleandstraightforward.Noultracentrifugationisrequired.
KitComponents:
•MitochondriaExtractionBuffer
•5XCytosolExtractionBuffer
•DTT(1M)
•500XProteaseInhibitorCocktail
•Anti-CytochromecmousemAb(0.2mg/ml).
Description:
Cytochromecplaysanimportantroleinapoptosis.Theproteinislocatedinthespacebetweentheinnerandoutermitochondrialmembranes.AnapoptoticstimulustriggersthereleaseofcytochromecfromthemitochondriaintocytosolwhereitbindstoApaf-1.Thecytochromec/Apaf-1complexactivatescaspase-9,whichthenactivatescaspase-3andotherdownstreamcaspases.CytochromecReleasingApoptosisAssayKitprovidesaneffectivemeansfordetectingcytochromectranslocationfrommitochondriaintocytosolduringapoptosis.Thekitprovidesuniqueformulationsofreagentstoisolateahighlyenrichedmitochondriafractionfromcytosol.Theprocedureissosimpleandeasytoperform,noultracentrifugationisrequiredandnotoxicchemicalsareinvolved.CytochromecreleasingfrommitochondriaintocytosolisthendeterminedbyWesternblottingusingthecytochromecantibodyprovidedinthekit.
StorageConditions:-20°C.
ShippingConditions:gelpack.
USAGE:ForResearchUseOnly!NotForUseinHumans.
