DNADamageQuantificationColorimetricKit.

KitSummary:

•Detectionmethod-Absorbance(450and650nm)

•Speciesreactivity-Mammalian

•Applications-KitprovidesallnecessaryreagentsforconvenientdeterminationofDNAdamage(abasicsites)insampleDNA.

SampleType:

PurifieddamagedDNA

Features&Benefits:

Kitprovidesallnecessaryreagentsfor96-wellplateformat.

KitComponents:

•ARPSolution

•TEBuffer

•GlycogenSolution

•0ARP-DNAStandard

•40ARP-DNAStandard

•DNABindingSolution

•HRP-Streptavidin

•10XWashBuffer

•HRPDeveloper

•96-wellMicroplate(8x12strips).

Description:

Apurinic/apyrimidinic(AP)sitesareoneofthemajortypesofDNAlesionsformedduringthecourseofbaseexcisionandrepairofoxidized,deaminatedoralkylatedbases.Ithasbeenestimatedthatabout2x105baselesionsaregeneratedpercellperday.ThelevelofAPsitesincellscanbeagoodindicatorofDNAlesionandrepairagainstchemicaldamageandcellaging.TheDNADamageQuantificationKitutilizestheARP(AldehydeReactiveProbe)reagentthatreactsspecificallywithanaldehydegroupwhichistheopenringformoftheAPsites.AftertreatingDNAcontainingAPsiteswithARPreagents,APsitesaretaggedwithbiotinresidues,whichcanbequantifiedusingavidin-biotinassayfollowedbyacolorimetricdetection.ThekitprovidesthenecessaryreagentsforconvenientdeterminationofabasicsitesinpurifiedDNAsamplein96-wellplateformat.

StorageConditions:+4°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.