GAPDHActivityAssayKit.

KitSummary:

•Detectionmethod-Absorbance(450nm)

•Applications-Measurementofglyceraldehyde-3-phosphatedehydrogenase(GAPDH)activityinvarioustissues/cells

-Analysisofglycolysisandpentosephosphatepathways.

SampleType:

•Animaltissues:Liver,Heartetc.

•Cellculture:AdherentorsUSPensioncells.

Features&Benefits:

•Simple,rapid&convenient

•CanmeasureGAPDHactivityaslowas100µU/mLinavarietyofsamples.

KitComponents:

•GAPDHAssayBuffer

•GAPDHSubstrate

•GAPDHDeveloper

•NADHStandard(500nmol)

•GAPDHPositiveControl

Description:

GAPDH(Glyceraldehyde-3-PhosphateDehydrogenase;EC1.2.1.12)catalyzestheconversionofGlyceraldehyde-3-Phosphate(GAP)to1,3-BisphosphateGlycerate(BPG)andplaysakeyroleinglycolysis.Theenzymeisinvolvedincellularprocessessuchasapoptosis,membranetrafficking,ironmetabolismandnucleartranslocation.GAPDH(housekeepinggene)expressionisstableandconstitutive.DeregulationofGAPDHactivityisassociatedwithabnormalcellproliferationandcarcinogenesis.AccuratequantitationofGAPDHactivityisimportantfordiagnosingdiseasesandstudyingnormalcellularphysiology.GAPDHActivityAssayKitprovidesasimpleandsensitivemethodformonitoringGAPDHactivityinvarioussamples.Inthisassay,GAPDHcatalyzesconversionofGAPintoBPGandanintermediate,whichreactswithadevelopertoformacoloredproductthatabsorbsmaximallyat450nm.Ourhigh-throughputadaptableassaycandetectGAPDHactivityaslowas100µU/mLinavarietyofsamples.

StorageConditions:-20°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.