GSTFluorometricActivityAssayKit.

Highlysensitiveinvitroassay

KitSummary:

•Detectionmethod-Fluorescence(Ex/Em380/461nm)

•Sampletype-CellandTissuelysates,culturemedia,urine,plasmaandserum,aswellasmanyotherBIOLOGicalfluids

•Speciesreactivity-Mammalian

•Applications-GSTlevelinsamplescanbeeasilydetectedusingafluorometerora96-wellfluorometricplatereader.ThekitcandetectGSTactivityincrudecelllysateorpurifiedproteinfraction,andalsoquantitateGST-taggedfusionprotein.Detects<0.5mU.

Features&Benefits:

•Simpleprocedure;takesonly~1-2hours

•Fastandconvenientp>

KitComponents:

•GSTAssayBuffer

•GSTSampleBuffer

•MCBSubstrate(inDMSO)

•Glutathione(lyophilized)

•GSTStandard(5mU/µl)

Description:

GlutathioneS-transferase(GST)isafamilyofenzymesthatplayanimportantroleindetoxificationofxenobiotics.GSTcatalyzestheformationofthethiolgroupofglutathionetoelectrophilicxenobiotics.Itutilizesglutathionetoscavengepotentiallytoxiccompoundsincludingthoseproducedasaresultofoxidativestressandispartofthedefensemechanismagainstthemutagenic,carcinogenicandtoxiceffectsofsuchcompounds.TheGSTFluorometricActivityAssayKitprovidesasimple,fluorescence-basedinvitroassayfordetectingtheGSTactivityusingfluorescenceplatereader.Theassayutilizesmonochlorobimane(MCB),adyethatreactswithglutathione.ThefreeformofMCBisalmostnonfluorescent,whereasthedyefluorescesblue(ex/em=380/461nm)whenreactswithglutathione.GSTcatalyzestheMCB-glutathionereactionsandthefluorescencelevelsareproportionallytotheamountsofGSTpresenceinthereaction.Thus,theGSTlevelinsamplescanbeeasilydetectedusingafluorometerora96-wellfluorometricplatereader.ThekitcandetectGSTactivityincrudecelllysateorpurifiedproteinfraction,andalsoquantitateGST-taggedfusionprotein.Detects<0.5mU.

StorageConditions:-20°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.