HATActivityFluorometricAssayKit.

SensitiveassaytomeasureHATactivity

KitSummary:

•Detectionmethod-Fluorescence(Ex/Em=535/587nm)

•Speciesreactivity-N/A

•Applications:

-MeasurementofHATactivityinNuclearExtracts

-MeasurementofHATactivityofpurifiedenzymepreparations.

SampleType:

•Nuclearextractsfromcellsandtissue

•Recombinantenzyme

Features&Benefits:

•Simple,rapid&convenient

•TheassaycandetectHATActivityaslowas6mUinavarietyofsamples

KitComponents:

•HATAssayBuffer

•AcetylCoA(Lyophilized)

•H3Peptide(Lyophilized)

•SubstrateMix(Lyophilized)

•Developer

•PicoProbe™

•CoAStandard(Lyophilized)

•PositiveControl(HeLaNuclearExtract)

Description:

HistoneAcetyltransferases(HATs)areenzymesthatacetylatehistonesubstratesresultinginimportantregulatoryeffectsonchromatinstructureandassembly,andgenetranscription.ModificationsoftheseproteinsbyHATsplayanimportantroleinthecontrolofgeneexpression,andtheirdysregulationhasbeenlinkedtocancer,neurodegeneration,andotherdiseases.HansABIoMed"sHATActivityAssayKitutilizesAcetylCoAandH3histonepeptideassubstrates.Inthisassay,HATenzymecatalyzesthetransferofacetylgroupsfromAcetyl-CoAtothehistonepeptide,therebygeneratingtwoproducts-acetylatedpeptideandCoA-SH.TheCoA-SHreactswiththedevelopertogenerateaproductthatisdetectedfluorometricallyatEx/Em=535/587nm.TheassaycandetectHATactivityaslowas6mUinavarietyofsamples.

StorageConditions:-80°C.

ShippingConditions: dryice.

USAGE:ForResearchUseOnly!NotForUseinHumans.