LipidPeroxidation(MDA)Colorimetric/FluorometricAssayKit.

Sensitive,Colorimetric&FluorometricAssay

KitSummary:

•Detectionmethod-Absorbance(532nm)orFluorescence(Ex/Em532/553nm)

•Sampletype-CellandTissueculturesupernatants,plasmaandotherBIOLOGicalfluids(optimizedbyenduser).

•Speciesreactivity-All

•Applications-ThekitdetectsMDAlevelsaslowas1nmol/wellcolorimetricallyand0.1nmol/wellfluorometrically.

Features&Benefits:

•Simpleprocedure

•Fastandconvenient

•Sensitiveassayformeasuringlipidperoxidation(LPA)inawiderangeofsamples.

KitComponents:

•MDALysisBuffer

•PhosphotungsticAcidSolution

•BHT(100X)

•TBA

•MDAStandard(4.17M)

Description:

Quantificationoflipidperoxidationisessentialtoassessoxidativestressinpathophysiologicalprocesses.LipidperoxidationformsMalondialdehyde(MDA)and4-hydroxynonenal(4-HNE),asnaturalbi-products.Measuringtheendproductsoflipidperoxidationisoneofthemostwidelyacceptedassaysforoxidativedamage.HansABIoMedLipidPeroxidationAssayKitprovidesaconvenienttoolforsensitivedetectionoftheMDAinavarietyofsamples.TheMDAinthesampleisreactedwithThiobarbituricAcid(TBA)togeneratetheMDA-TBAadduct.TheMDA-TBAadductcanbeeasilyquantifiedcolorimetrically(λ=532nm)orfluorometrically(Ex/Em=532/553nm).ThisassaydetectsMDAlevelsaslowas1nmol/wellcolorimetricallyand0.1nmol/wellfluorometrically.

StorageConditions:-20°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.