GlutathioneReductaseActivityColorimetricAssayKit.

HighlySensitiveAssay,HTS

KitSummary:

•Detectionmethod-Absorbance(405nm)

•Sampletype-CellandTissueculturesupernatants,urine,plasmaandserum,erythrocytelysates,aswellasmanyotherBIOLOGicalfluidsetc.

•Speciesreactivity-Mammalian

•Application-Theassaycandetect0.1–40mU/mlGRinvarioussamples.

Features&Benefits:

•Simpleprocedure;takes~30-40minutes

•Fastandconvenient

KitComponents:

•GRAssayBuffer

•3%H₂O₂

•Catalase

•TNBStandard(5mM)

•DTNB

•NADPH-GNERAT™

•GSSG

•GRPositiveControl(10mU)

Description:

GlutathioneReductase(GR,EC1.8.1.7)catalyzestheNADPH-dependentreductionofoxidizedglutathione(GSSG)toreducedglutathione(GSH),whichplaysanimportantroleintheGSHredoxcyclethatmaintainsadequatelevelsofreducedGSH.AhighGSH/GSSGratioisessentialforprotectionagainstoxidativestress.HansABIoMed’sGlutathioneReductaseAssayKitisahighlysensitive,simple,directandHTS-readycolorimetricassayformeasuringGRactivityinbiologicalsamples.Intheassay,GRreducesGSSGtoGSH,whichreactswith5,5′-Dithiobis(2-nitrobenzoicacid)(DTNB)togenerateTNB2-(yellowcolor,λmax=405nm).Theassaycandetect0.1–40mU/mlGRinvarioussamples.

StorageConditions:-20°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.