>
产品中心 >
acid_base_buffer_solution >
HansaBioMed/Alanine Aminotransferase (ALT or SGPT) Assay Kit (100 reactions)/HBM-K752-100/1 Ea
AlanineAminotransferase(ALTorSGPT)ActivityColorimetric/FluorometricAssayKit.
SensitiveAssay,HTS
KitSummary:
•Detectionmethod-Absorbance(570nm)orFluorescence(Ex/Em535/587nm)
•Sampletype-CellandTissueculturesupernatants,urine,plasmaandserum,aswellasmanyotherBIOLOGicalfluids,growthmedium,foodetc.
•Speciesreactivity-Mammalian
•Applications-ThekitdetectsALTwithadetectionlimitof10mUperwell.
Features&Benefits:
•Simpleprocedure
•Fastandconvenient
•SensitiveassaysformeasuringALTactivityinvariousbiologicalsamplesandthekitisalsosuitableforhighthroughputanalyses.
KitComponents:
•ALTAssayBuffer
•OxiRedProbe(inDMSO)
•ALTEnzymeMix
•ALTSubstrate
•PyruvateStandard(100nmol/µl)
•ALTpositivecontrol
Description:
Alanineaminotransferase(ALT)isatransaminaseenzyme(EC2.6.1.2).Itisalsocalledserumglutamicpyruvictransaminase(SGPT)oralaninetransaminase(ALAT).ALTisfoundinserumandinvariousbodilytissues,butisusuallyassociatedwiththeliver.Itcatalyzesthereaction:
α-ketoglutarate+alanine⇌glutamate+pyruvate.
Itiscommonlymeasuredclinicallyasapartofadiagnosticliverfunctiontesttodetermineliverhealth.Diagnostically,itisalmostalwaysmeasuredinunits/liter(U/L).InALTAssayKit,ALTcatalyzesthetransferofanaminogroupfromalanineto⍺-ketoglutarate,theproductsofthisreversIBLetransaminationreactionbeingpyruvateandglutamate.Thepyruvateisdetectedinareactionthatconcomitantlyconvertsanearlycolorlessprobetobothcolor(λmax=570nm)andfluorescence(Ex/Em=535/587nm).Thekitprovidesarapid,simple,sensitive,andreliabletestsuitableforhighthroughputactivityassayofALTwithadetectionlimitof10mUperwell.
StorageConditions:-20°C.
ShippingConditions:gelpack.
USAGE:ForResearchUseOnly!NotForUseinHumans.
