β-Hydroxybutyrate(β-HB)ColorimetricAssayKit.

Sensitive,ColorimetricAssay

KitSummary:

•Detectionmethod-Absorbance(450nm)

•Sampletype-CellandTissueculturesupernatants,urine,plasmaandserum,aswellasmanyotherBIOLOGicalfluids

•Speciesreactivity-Mammalian

•Application-Theassayislinearfor1-20nmolβ-HBinupto100µlsamplesor0.01-0.2mMofβ-HBsamples.

Features&Benefits:

•Simpleprocedure;takes~40minutes

•Fastandconvenient

•Thekitprovidesanaccurateassayformeasuringβ-Hydroxybutyrate(β-HB)invarioussample.

KitComponents:

•β-HBAssayBuffer

•β-HBEnzymeMix

•β-HBSubstrateMix

•β-HBStandard(1.0µmol).

Description:

Diabeticketoacidosisoccurswhencirculatinginsulinlevelsdroptoverylowlevels,shuttingoffthesupplyofglucosetothebody.Thephysiologicalresponseisforthelivertoproduceketonebodies(acetoacetate,acetone,andprimarilyβ-hydroxybutyrate)fromtheacetylCoAproducedfromfattyacidoxidation.Theveryhighrateofketonebodyproductionoutstripsthebody’sABIlitytoutilizethemasanenergysourceandthebloodconcentrationbuildsup.Asratherstrongacids,theyresultinasignificantdropinbloodpH.HansaBioMed’sβ-HBAssaykitutilizesβ-HBDehydrogenasetogenerateaproductwhichreactswithourcolorimetricprobewithanabsorbancebandat450nm.Thekitisaneasyandaccurateassaytomeasureβ-HBlevelsinbiologicalsamples.Theassayislinearfor1-20nmolβ-HBinupto100µlsamplesor0.01-0.2mMofβ-HBsamples.

StorageConditions:-20°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.