Beta-SecretaseActivityFluorometricAssayKit.

HighlySensitiveAssay

KitSummary:

•Detectionmethod-Fluorescence(Ex/Em335-355/495-510nm)

•Sampletype-CellandTissuelysates,culturemedia,urine,plasmaandserum,aswellasmanyotherBIOLOGicalfluids

•Speciesreactivity-Mammalian

•Applications-Convenientfluorescencemethodfordetectingβ-secretaseactivityinbiologicalandpurifiedsamples.

Features&Benefits:

•Simpleprocedure;takes~1hour

•Fastandconvenient

•Theassayissensitive,stableandhigh-throughputadaptable.

KitComponents:

•β-SecretaseExtractionBuffer

•β-SecretaseReactionBuffer(2X)

•β-SecretaseSubstrate(inDMSO)

•Activeβ-Secretase(Lyophilized)

•β-SecretaseInhibitor(inDMSO)

Description:

β-Secretasehasbeenimplicatedtobeanexcellenttargetforanti-amyloidtherapyforthetreatmentofAlzheimer’sdisease.Theβ-SecretaseactivityAssayKitprovidesaconvenientfluorescencemethodfordetectingβ-secretaseactivityinbiologicalandpurifiedsamples.Theassayutilizesasecretase-specificpeptideconjugatedtotworeportermoleculesEDANSandDABCYL.Intheuncleavedform,thefluorescentemissionsfromEDANSarequenchedbythephysicalproximityoftheDABCYLmoiety.CleavageofthepeptidebysecretasephysicallyseparatesEDANSandDABCYLallowingforthereleaseofafluorescentsignal.Thelevelofsecretaseenzymaticactivityinsamplesisproportionaltotheleveloffluorescenceintensity.

StorageConditions:-20°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.