N′-NicotinamideMethyltransferase(NNMT)InhibitorScreeningAssayKit.

TheFIRSTandONLYkitonthemarketforNicotinamideN-Methyltransferase(NNMT)InhibitorScreening

KitSummary:

•Detectionmethod-Fluorescence(Ex/Em=392/482nm)

•Speciesreactivity-Mammalian

•Application-Screening/studying/characterizingpotentialinhibitorsofNNMT.

Features&Benefits:

•Rapidassayandsimpleprotocol

•ReliabletestforscreeningpotentialinhibitorsofNNMT

•IncludesInhibitorControl,1-Methylnicotinamide(MNA)

KitComponents:

•NNMTAssayBuffer

•NNMTEnzyme

•S-Adenosylmethionine(SAM)

•Nicotinamide

•Enzyme-I

•Enzyme-II

•1-Methylnicotinamide(MNA)(150mM)

•ThiolDetectingProbe(DMSO)

•SAMReconstitutionBuffer

Description:

N′-NicotinamideMethyltransferase(NNMT)(E.C.2.1.1.1)catalyzestheN-methylationofnicotinamide,pyridines,andotheranaloguesusingS-adenosylmethionine(SAM)asthedonorresultingintheproductionof1-methylnicotinamide(MNA).NNMTplaysasignificantroleintheregulationofmetabolicpathwaysandisexpressedatmarkedlyhighlevelsinseveralkindsofcancers,neurodegenerativediseases,obesityanddiabetes,indicatingitisapotentialmoleculartargetfortherapy.TheNNMTinhibitorscreeningkitutilizesSAMasthemethylgroupdonorandnicotinamideasthesubstrate.NNMTmethylatesnicotinamidegeneratingS-adenosylhomocysteine(SAH)and1-methylnicotinamide.TheSAHishydrolyzedbySAHhydrolasetoformhomocysteine,thefreethiolgroupofwhichisdetectedusingaThiolDetectingProbegeneratingenhancedfluorescencesignalthatcanbemeasuredatEx/Em=392/482nm.InthepresenceofanNNMTinhibitor,theenzymaticactivityisinhibitedresultingindecreasedfluorescence.Thisassaykitisasimple,sensitive,andrapidtooltoscreenpotentialinhibitorsofNNMT.

StorageConditions:-80°C.

ShippingConditions:dryice.

USAGE:ForResearchUseOnly!NotForUseinHumans.