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HansaBioMed/Branched Chain Amino Acid Kit (100 reactions)/HBM-K564-100/1 Ea
BranchedChainAminoAcid(Leu/Ile/Val)ColorimetricAssayKit.
Sensitive,ColorimetricAssay
KitSummary:
•Detectionmethod-Absorbance(450nm)
•Sampletype-CellandTissueculturesupernatants,urine,plasmaandserum,aswellasmanyotherBIOLOGicalfluids
•Speciesreactivity-Mammalian
•Applications-HansABIoMed’sBCAAkitmeasuresBCAA’sintherangeof0to10nmolpersamplewithadetectionlimitof~0.2nmol(~10µMBCAAinsample).
Features&Benefits:
•Simpleprocedure;takeslessthan~40minutes
•Fastandconvenient
KitComponents:
•BCAAAssayBuffer
•BCAAEnzymeMix
•WSTSubstrateMix
•LeuStandard(1µmol)
Description:
Thebranched-chainaminoacidsorBCAA’s,refertotheaminoacidswithnon-linearaliphaticside-chains,namelyleucine,isoleucineandvaline.Thesethreeessentialaminoacidsmakeupapproximately1/3ofskeletalmuscleinthehumanbody.BCAA’sarecurrentlyusedclinicallytoaidintherecoveryofburnvictims,aswellasforstrengthsupplementationforathletes.BCAA’s,primarilyLeu,canstimulateinsulinsecretion.TheBCAA’shavealsobeenimplicatedinawiderangeofotherphysiologicaleffects.HansaBioMed"sBCAAAssayKitprovidesasimpleconvenientmeansofmeasuringtheBCAA’sinavarietyofbiologicalsamples.ThekitutilizesanenzymeassayinwhichBCAAisoxidativelydeaminated,producingNADHwhichreducestheprobe,generatingacoloredproduct(λmax=450nm).TheBCAAkitmeasuresBCAA’sintherangeof0to10nmolpersamplewithadetectionlimitof~0.2nmol(~10µMBCAAinsample).BCAA’sarepresentinserum~0.1-0.4mMeach(~0.125-1.5mMcombined).
StorageConditions:-20°C.
ShippingConditions:gelpack.
USAGE:ForResearchUseOnly!NotForUseinHumans.