GlutathioneColorimetricAssayKit.

DetectseithertotalglutathioneorexclusivelyGSH

KitSummary:

•Detectionmethod-Absorbance(412nm)

•Sampletype-CellandTissuelysates,culturemedia,urine,plasmaandserum,aswellasmanyotherBIOLOGicalfluids

•Speciesreactivity-Mammalian

•Applications-TheColorimetricGlutathioneDetectionKitprovidesasimpleinvitroassayfordetectingtheGSHchangesinapoptosisandotherpathologicalprocesses.

Features&Benefits:

•Simpleprocedure;takesonly~2-3hours

•Fastandconvenient

KitComponents:

•GlutathioneReactionBuffer

•GlutathioneSubstrate(DTNB)

•NADPHGeneratingMix(lyophilized)

•GlutathioneReductase(lyophilized)

•SulfosalicylicAcid(SSA,1gram)

•GSHStandard(lyophilized,MW307)

Description:

Glutathione(GSH)isthemajorintracellularlow-molecular-weightthiolthatplaysacriticalroleinthecellulardefenseagainstoxidativestressinmammaliancells.HansABIoMed’sApoGSH™GlutathioneColorimetricAssayKitprovidesaconvenient,colorimetricmethodforanalyzingeithertotalglutathioneorthereducedformglutathionealoneusingamicrotiterplatereader.TheassayisbasedontheglutathionerecyclingsystembyDTNBandglutathionereductase(Fig.1).DTNBandglutathione(GSH)reacttogenerate2-nitro-5-thiobenzoicacidwhichhasyellowcolor.Therefore,GSHconcentrationcanbedeterminedbymeasuringabsorbanceat412nm.ThegeneratedGSSGcanbereducedbacktoGSHbyglutathionereductase,andGSHreactswithDTNBagaintoproducemore2-nitro-5-thiobenzoicacid.Therefore,therecyclingsystemdramaticallyimprovesthesensitivityoftotalglutathionedetection.Thekitincludesthe5-Sulfosalicylicacid(SSA)fortheremovalofproteinsfromsamplesandfortheprotectionofGSHoxidationandγ-glutamyltranspeptidasereaction.Thekitcanquantifyglutathionefrom1-100ng/wellina200lreaction.Fordetectinglowerglutathioneconcentrations,suchasinbloodsamples,increasingreactiontimewillgeneratestrongersignal.Thekitcanalsospecificallydetectthereducedformofglutathione(GSH)byomittingtheglutathionereductasefromthereactionmixture.Thesensitivityfordetectingthereducedformofglutathione(withoutrecyclingsystem)is100timeslowerthandetectingthetotalglutahione.

StorageConditions:-20°C.

ShippingConditions:gelpack.

USAGE:ForResearchUseOnly!NotForUseinHumans.