DeproteinizingSamplePreparationKit.

SimpleKittodeproteinizesamples

KitSummary:

•Sampletype-CellandTissueculturehomogenates(eukaryoticcells),serum,plasmaandhighproteinsamples

•Speciesreactivity-Mammalian

•Application-Uniquetooltopreparesamplesfortheanalysisofavarietyofsmallmolecules,proteinsareprecipitated,excessPCAisremoved,samplesareneutralized.

Features&Benefits:

•Simpleprocedure;takes~20minutes

•Fastandconvenient

•Samplespreparedusingthiskitcanbedirectlyusedinawidevarietyofsmallmoleculebioassays.

KitComponents:

•PCA

•NeutralizationSolution

Description:

ThepresenceofproteinandvariousenzymeactivitiesfrequentlyinterfereswiththeanalysisofsmallmoleculesinBIOLOGicalsamples.Manybioassaysrequireremovalofproteinsfromsamplespriortoanalysis.Amongthedeproteinizationprotocolsdevelopedoverthelasthalfcentury,perchloricacid(PCA)precipitationhasbeenextensivelyusedinmanydifferentsamplepreparationprocedures,sincenotonlydoesitremovemostoftheproteinpresentbutitalsofunctionstostABIlizemanyofthesmallmoleculeanalytes.PCAdeproteinizationmethodshavebeensuccessfullyusedinthepreparationsamplespriortoquantitationofanarrayofsmallmolecules,includingglycogen,ATP,cAMP,glutathione,antioxidants,etc.ThenewlydesignedDeproteinizingSamplePreparationKitutilizesaPCAprecipitationmethod,providingauniquetooltopreparesamplesfortheanalysisofavarietyofsmallmolecules.Usingthekit,proteinsareprecipitated,excessPCAisremoved,samplesareneutralized.Samplespreparedusingthiskitcanbedirectlyusedinawidevarietyofbioassays.Themethodiseasy,convenient,andcanbeusedforthepreparationofalargenumberofsamples.

StorageConditions:RoomTemperature.

ShippingConditions:RoomTemperature.

USAGE:ForResearchUseOnly!NotForUseinHumans.