GAPDH Activity Assay Kit.

Kit Summary:

• Detection method - Absorbance (450 nm)

• Applications - Measurement of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity in various tissues/cells

- Analysis of glycolysis and pentose phosphate pathways.

Sample Type:

• Animal tissues: Liver, Heart etc.

• Cell culture: Adherent or suspension cells.

Features & Benefits:

• Simple, rapid & convenient

• Can measure GAPDH activity as low as 100 µU/mL in a variety of samples.

Kit Components:

• GAPDH Assay Buffer

• GAPDH Substrate

• GAPDH Developer

• NADH Standard (500 nmol)

• GAPDH Positive Control

Description:

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase; EC 1.2.1.12) catalyzes the conversion of Glyceraldehyde-3-Phosphate (GAP) to 1, 3-Bisphosphate Glycerate (BPG) and plays a key role in glycolysis. The enzyme is involved in cellular processes such as apoptosis, membrane trafficking, iron metabolism and nuclear translocation. GAPDH (housekeeping gene) expression is stable and constitutive. Deregulation of GAPDH activity is associated with abnormal cell proliferation and carcinogenesis. Accurate quantitation of GAPDH activity is important for diagnosing diseases and studying normal cellular physiology. GAPDH Activity Assay Kit provides a simple and sensitive method for monitoring GAPDH activity in various samples. In this assay, GAPDH catalyzes conversion of GAP into BPG and an intermediate, which reacts with a developer to form a colored product that absorbs maximally at 450 nm. Our high-throughput adaptable assay can detect GAPDH activity as low as 100 µU/mL in a variety of samples.

Storage Conditions: -20°C.

Shipping Conditions: gel pack.

USAGE: For Research Use Only! Not For Use in Humans.